Isolation and mapping of a polymorphic DNA sequence (pTHH33) on chromosome 1q [D1S81]
نویسندگان
چکیده
منابع مشابه
Isolation and mapping of a polymorphic DNA sequence (pMCT96.1 and pMCT96.2) on chromosome 9q [D9S14].
SOURCE/DESCRIPTION; A 4.5 kb (pMCT96.1) or a 3.7 kb (pMCT96.2) of Mspl fragment from cosmid MCT96 isolated by an oligonucleotide (GTGTGTGTGTGTGTGTGTGT) (1) was subcloned into the Accl site of pUC18. POLYMORPHISM: pMCT96.1 : Rsal identifies 3 systems of site polymorphisms; system 1: 4.7 kb (Rl) or 3.7 kb (R2); system 2: 3.0 (R3) kb or 2.8 kb (R4); system 3: 1.0 kb (R5) or 0.9 kb (R6) pMCT96.2 : ...
متن کاملIsolation and mapping of a polymorphic DNA sequence (pEFD134.7) on chromosome 3 [D3S45].
SOURCE/DESCRIPTION; A 4.3 lib Mspl fragment of cosmid EFD134 isolated by a HBV-3 oligonucleotide (1) was subcloned into AccI site of pUC18. POLYMORPHISM; Mspl identifies >10 allelic VNTR polymorphism with bands between 1.5 and 2.7 kb. Rsal and TaqI also identify the same VNTR polymorphism. FREQUENCY: With Mspl, 80 % heterozygosity were observed in 85 unrelated Caucasians. NOT POLYMORPHIC FOR: n...
متن کاملIsolation and mapping of a polymorphic DNA sequence pYNZ86.1 on chromosome 3 (D3S30).
SOURCE/DESCRIPTION: A 3.3 kb Hindi 11 fragment from cosmid YNZ86 was subcloned into the Hindlll site of pUClB. POLYMORPHISM: Mspl identifies a two allele polymorphism (Ml : 2.0 kb, M2 : 1.9 kb) with constant bands at 2.6 and 1.7 kb. PvuII also identifies a two allele polymorphism (PI : 1.1 kb, P2 : 0.8 kb) with a constant band at 1.5 kb, but this observation has not been verified by pedigree st...
متن کاملIsolation and mapping of a polymorphic DNA sequence (cMCOD13) on chromosome 3 [D3S44].
SOURCE/DESCRIPTION; This cosmid HCOD13 has been isolated by an oligonucleotide (1) from a human genomic cosmid library. POLYMORPHISM: PvuII identifies >10 allelic VNTR polymorphisms with bands between 4.0 and 10.0 kb. TaqI, Rsal Mspl and Pstl identify the same VNTR polymorphism.
متن کاملIsolation and mapping of a polymorphic DNA sequence pYNH24 on chromosome 2 (D2S44).
SOURCE/DESCRIPTION; A 2.0 kb Mspl fragment from cosmid YNH24 isolated by HBV-2 oligonucleotide (GGAGTTGGGGGAGGAG) (1) was subcloned into the AccI site of pUC18. POLYMORPHISM: Mspl identifies a >30 allele VNTR polymorphism with bands between 1.0-5.0 kb. Taql, Bglll, Pvull, PstI and BamHI also detect the same polymorphism.
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1988
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/16.19.9363